Enzymatic hydrolysis of marine fish gelatin for producing ACE inhibitor peptides: meta-analysis

Bioactive peptides showing Angiotensin-I-Converting Enzyme inhibitory (ACEi) activity can control blood pressure. They be produced from various protein sources, including fish gelatin. This article provides a systematic review and meta-analysis on gelatin extraction, conditions of enzymatic hydrolysis, characteristics ACEi peptides, effect hydrolysis procedure peptides. Fish preparation uses solutions such as acids, bases distilled water. Enzymatic is carried out with an E/S ratio 0.2 – 7% temperature 28.9 oC (using bromelain) 60.6 Alcalase). Level pH in also varied greatly, 2 Pepsin) to 10 Purafect enzyme). The molecular weight ranged 186-829 Da the sequence was dominated by hydrophobic aliphatic amino acids. Based meta-analysis, using enzyme combination resulted more satisfying product than single enzyme, represented combined size value 0.593.